On-line liquid chromatography/tandem mass spectrometry simultaneous determination of opiates, cocainics and amphetamines in dried blood spots.
Identifieur interne : 000558 ( France/Analysis ); précédent : 000557; suivant : 000559On-line liquid chromatography/tandem mass spectrometry simultaneous determination of opiates, cocainics and amphetamines in dried blood spots.
Auteurs : E. Saussereau [France] ; C. Lacroix ; J M Gaulier ; J P GoulleSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1873-376X ] ; 2012.
English descriptors
- KwdEn :
- Amphetamines (blood), Blood Specimen Collection (methods), Chromatography, Liquid (methods), Cocaine (blood), Dried Blood Spot Testing (methods), Drug Stability, Humans, Morphine Derivatives (blood), Reproducibility of Results, Sensitivity and Specificity, Street Drugs (blood), Tandem Mass Spectrometry (methods).
- MESH :
Abstract
A novel approach has been developed for the illicit drugs quantitative determination using dried blood spots (DBS) on filter paper. The illicit drugs tested were opiates (morphine and its 3- and 6-glucuronide metabolites, codeine, 6-monoacetylmorphine), cocainics (ecgonine methylester, benzoylecgonine, cocaine, cocaethylene) and amphetamines (amphetamine, methamphetamine, MDA, MDMA, MDEA). The described method, requiring a small blood volume, is based on high performance liquid chromatography coupled to tandem mass spectrometry using on-line extraction. A Whatman card 903 was spotted with 30μL of whole blood and left overnight to dry at room temperature. A 3-mm diameter disk was removed using a manual punch, suspended in 150μL of water for 10min with ultrasonication, and then 100μL was injected in the on-line LC-MS/MS system. An Oasis HLB was used as an extraction column and a C18 Atlantis as an analytical column. The chromatographic cycle was performed with 20mM ammonium formate buffer (pH 2.8) (solvent A) and acetonitrile/solvent A (90:10, v/v) gradient in 16min. Detection was performed in positive electrospray ionization mode (ESI+) with a Quattro Micro (Waters). Recoveries of all analytes were up to 80%. DBS were stored in duplicate at 4°C and -20°C for up to 6 months. Illicit drugs seemed to be much more stabled at -20°C. Furthermore, it was tested whether analysis of DBS may be as reliable as that of whole blood investigating authentic samples; significant correlations were obtained. This DBS assay has potential as rapid, sensitive and inexpensive option for the illicit drugs determination in small blood volumes, which seems of great interest in suspected cases of driving under the influence of drugs.
DOI: 10.1016/j.jchromb.2011.11.035
PubMed: 22281234
Affiliations:
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pubmed:22281234Le document en format XML
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Saussereau</name><affiliation wicri:level="3"><nlm:affiliation>Pharmacokinetic and Toxicology Laboratory, J. Monod Hospital, F-76083 Le Havre Cedex, France. esaussereau@ch-havre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Pharmacokinetic and Toxicology Laboratory, J. Monod Hospital, F-76083 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Lacroix, C" sort="Lacroix, C" uniqKey="Lacroix C" first="C" last="Lacroix">C. Lacroix</name></author><author><name sortKey="Gaulier, J M" sort="Gaulier, J M" uniqKey="Gaulier J" first="J M" last="Gaulier">J M Gaulier</name></author><author><name sortKey="Goulle, J P" sort="Goulle, J P" uniqKey="Goulle J" first="J P" last="Goulle">J P Goulle</name></author></analytic><series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><idno type="eISSN">1873-376X</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amphetamines (blood)</term><term>Blood Specimen Collection (methods)</term><term>Chromatography, Liquid (methods)</term><term>Cocaine (blood)</term><term>Dried Blood Spot Testing (methods)</term><term>Drug Stability</term><term>Humans</term><term>Morphine Derivatives (blood)</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Street Drugs (blood)</term><term>Tandem Mass Spectrometry (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Amphetamines</term><term>Cocaine</term><term>Morphine Derivatives</term><term>Street Drugs</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Blood Specimen Collection</term><term>Chromatography, Liquid</term><term>Dried Blood Spot Testing</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Drug Stability</term><term>Humans</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel approach has been developed for the illicit drugs quantitative determination using dried blood spots (DBS) on filter paper. The illicit drugs tested were opiates (morphine and its 3- and 6-glucuronide metabolites, codeine, 6-monoacetylmorphine), cocainics (ecgonine methylester, benzoylecgonine, cocaine, cocaethylene) and amphetamines (amphetamine, methamphetamine, MDA, MDMA, MDEA). The described method, requiring a small blood volume, is based on high performance liquid chromatography coupled to tandem mass spectrometry using on-line extraction. A Whatman card 903 was spotted with 30μL of whole blood and left overnight to dry at room temperature. A 3-mm diameter disk was removed using a manual punch, suspended in 150μL of water for 10min with ultrasonication, and then 100μL was injected in the on-line LC-MS/MS system. An Oasis HLB was used as an extraction column and a C18 Atlantis as an analytical column. The chromatographic cycle was performed with 20mM ammonium formate buffer (pH 2.8) (solvent A) and acetonitrile/solvent A (90:10, v/v) gradient in 16min. Detection was performed in positive electrospray ionization mode (ESI+) with a Quattro Micro (Waters). Recoveries of all analytes were up to 80%. DBS were stored in duplicate at 4°C and -20°C for up to 6 months. Illicit drugs seemed to be much more stabled at -20°C. Furthermore, it was tested whether analysis of DBS may be as reliable as that of whole blood investigating authentic samples; significant correlations were obtained. This DBS assay has potential as rapid, sensitive and inexpensive option for the illicit drugs determination in small blood volumes, which seems of great interest in suspected cases of driving under the influence of drugs.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Gaulier, J M" sort="Gaulier, J M" uniqKey="Gaulier J" first="J M" last="Gaulier">J M Gaulier</name><name sortKey="Goulle, J P" sort="Goulle, J P" uniqKey="Goulle J" first="J P" last="Goulle">J P Goulle</name><name sortKey="Lacroix, C" sort="Lacroix, C" uniqKey="Lacroix C" first="C" last="Lacroix">C. Lacroix</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Saussereau, E" sort="Saussereau, E" uniqKey="Saussereau E" first="E" last="Saussereau">E. 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